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rap1gap crispr activation plasmid  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology rap1gap crispr activation plasmid
    A . Slides without primary antibody served as the negative control in GC tissues, B, E . Typical immunohistologic features with <t>Rap1GAP</t> expression in para-carcinoma and GC tissues, the Rap1GAP staining localized predominantly in the cytoplasm. C, F . Typical immunohistologic features with E-cadherin expression in para-carcinoma and GC tissues. D . Immunostaining for MMP2 was performed in GC; Magnifications, ×200.
    Rap1gap Crispr Activation Plasmid, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rap1gap+crispr+activation+plasmid/Rap1GAP+CRISPR+Activation+Plasmid/pmc05352382-216-11-15
    Average 86 stars, based on 1 article reviews
    rap1gap crispr activation plasmid - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "Low expression of Rap1GAP is associated with epithelial-mesenchymal transition (EMT) and poor prognosis in gastric cancer"

    Article Title: Low expression of Rap1GAP is associated with epithelial-mesenchymal transition (EMT) and poor prognosis in gastric cancer

    Journal: Oncotarget

    doi: 10.18632/oncotarget.14074

    A . Slides without primary antibody served as the negative control in GC tissues, B, E . Typical immunohistologic features with Rap1GAP expression in para-carcinoma and GC tissues, the Rap1GAP staining localized predominantly in the cytoplasm. C, F . Typical immunohistologic features with E-cadherin expression in para-carcinoma and GC tissues. D . Immunostaining for MMP2 was performed in GC; Magnifications, ×200.
    Figure Legend Snippet: A . Slides without primary antibody served as the negative control in GC tissues, B, E . Typical immunohistologic features with Rap1GAP expression in para-carcinoma and GC tissues, the Rap1GAP staining localized predominantly in the cytoplasm. C, F . Typical immunohistologic features with E-cadherin expression in para-carcinoma and GC tissues. D . Immunostaining for MMP2 was performed in GC; Magnifications, ×200.

    Techniques Used: Negative Control, Expressing, Staining, Immunostaining

    Differences in Rap1GAP, E-cadherin and MMP2 between the cancer tissues and para-carcinoma tissues
    Figure Legend Snippet: Differences in Rap1GAP, E-cadherin and MMP2 between the cancer tissues and para-carcinoma tissues

    Techniques Used:

    Rap1GAP and E-cadherin protein in GC cells were notably lower expression compared to 293T cells by western blotting. Conversely, MMP2 was upregulated in GC cells (*P < 0.05 and **P < 0.01)
    Figure Legend Snippet: Rap1GAP and E-cadherin protein in GC cells were notably lower expression compared to 293T cells by western blotting. Conversely, MMP2 was upregulated in GC cells (*P < 0.05 and **P < 0.01)

    Techniques Used: Expressing, Western Blot

    Correlations between the clinicopathologic variables with Rap1GAP, E-cadherin and MMP2
    Figure Legend Snippet: Correlations between the clinicopathologic variables with Rap1GAP, E-cadherin and MMP2

    Techniques Used:

    Association between Rap1GAP with E-cadherin and MMP2
    Figure Legend Snippet: Association between Rap1GAP with E-cadherin and MMP2

    Techniques Used:

    The cumulative overall survival differences between the patients with high and low levels of protein expression. The P value was obtained using the log-rank test of the difference. A . Rap1GAP; B . E-cadherin; C . MMP2.
    Figure Legend Snippet: The cumulative overall survival differences between the patients with high and low levels of protein expression. The P value was obtained using the log-rank test of the difference. A . Rap1GAP; B . E-cadherin; C . MMP2.

    Techniques Used: Expressing

    Univariate Analysis for Overall Survival
    Figure Legend Snippet: Univariate Analysis for Overall Survival

    Techniques Used:

    Multivariate Cox Proportional Hazards Analysis for Overall Survival
    Figure Legend Snippet: Multivariate Cox Proportional Hazards Analysis for Overall Survival

    Techniques Used:

    The GC cells were grown and transfected with the negative control (NC) or Rap1GAP CRISPR Activation Plasmid. Overexpression Rap1GAP improved the expression of E-cadherin (A, B) and, inversely, suppressed the expression of MMP2 (A, B) compared to NC group in mRNA level and protein level. (*P < 0.05 and **P < 0.01).
    Figure Legend Snippet: The GC cells were grown and transfected with the negative control (NC) or Rap1GAP CRISPR Activation Plasmid. Overexpression Rap1GAP improved the expression of E-cadherin (A, B) and, inversely, suppressed the expression of MMP2 (A, B) compared to NC group in mRNA level and protein level. (*P < 0.05 and **P < 0.01).

    Techniques Used: Transfection, Negative Control, CRISPR, Activation Assay, Plasmid Preparation, Over Expression, Expressing

    Overexpression Rap1GAP repressed the migration A . and invasion B . capacity of GC cells C . The graph shows the quantified data of the assay, migration (left) and invasion (right) (**P < 0.01).
    Figure Legend Snippet: Overexpression Rap1GAP repressed the migration A . and invasion B . capacity of GC cells C . The graph shows the quantified data of the assay, migration (left) and invasion (right) (**P < 0.01).

    Techniques Used: Over Expression, Migration

    Related Articles

    Transfection:

    Article Title: Low expression of Rap1GAP is associated with epithelial-mesenchymal transition (EMT) and poor prognosis in gastric cancer
    Article Snippet: .. The GC cells were grown and transfected with the NC or Rap1GAP CRISPR Activation Plasmid (Santa Cruz) using Lipofectamine 2000, according to the manufacturer's protocol. ..

    CRISPR:

    Article Title: Low expression of Rap1GAP is associated with epithelial-mesenchymal transition (EMT) and poor prognosis in gastric cancer
    Article Snippet: .. The GC cells were grown and transfected with the NC or Rap1GAP CRISPR Activation Plasmid (Santa Cruz) using Lipofectamine 2000, according to the manufacturer's protocol. ..

    Activation Assay:

    Article Title: Low expression of Rap1GAP is associated with epithelial-mesenchymal transition (EMT) and poor prognosis in gastric cancer
    Article Snippet: .. The GC cells were grown and transfected with the NC or Rap1GAP CRISPR Activation Plasmid (Santa Cruz) using Lipofectamine 2000, according to the manufacturer's protocol. ..

    Plasmid Preparation:

    Article Title: Low expression of Rap1GAP is associated with epithelial-mesenchymal transition (EMT) and poor prognosis in gastric cancer
    Article Snippet: .. The GC cells were grown and transfected with the NC or Rap1GAP CRISPR Activation Plasmid (Santa Cruz) using Lipofectamine 2000, according to the manufacturer's protocol. ..



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    rap1gap crispr activation plasmid  (Santa Cruz Biotechnology)
    86
    Santa Cruz Biotechnology rap1gap crispr activation plasmid
    A . Slides without primary antibody served as the negative control in GC tissues, B, E . Typical immunohistologic features with <t>Rap1GAP</t> expression in para-carcinoma and GC tissues, the Rap1GAP staining localized predominantly in the cytoplasm. C, F . Typical immunohistologic features with E-cadherin expression in para-carcinoma and GC tissues. D . Immunostaining for MMP2 was performed in GC; Magnifications, ×200.
    Rap1gap Crispr Activation Plasmid, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rap1gap+crispr+activation+plasmid/Rap1GAP+CRISPR+Activation+Plasmid/pmc05352382-216-11-15
    Average 86 stars, based on 1 article reviews
    rap1gap crispr activation plasmid - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    A . Slides without primary antibody served as the negative control in GC tissues, B, E . Typical immunohistologic features with Rap1GAP expression in para-carcinoma and GC tissues, the Rap1GAP staining localized predominantly in the cytoplasm. C, F . Typical immunohistologic features with E-cadherin expression in para-carcinoma and GC tissues. D . Immunostaining for MMP2 was performed in GC; Magnifications, ×200.

    Journal: Oncotarget

    Article Title: Low expression of Rap1GAP is associated with epithelial-mesenchymal transition (EMT) and poor prognosis in gastric cancer

    doi: 10.18632/oncotarget.14074

    Figure Lengend Snippet: A . Slides without primary antibody served as the negative control in GC tissues, B, E . Typical immunohistologic features with Rap1GAP expression in para-carcinoma and GC tissues, the Rap1GAP staining localized predominantly in the cytoplasm. C, F . Typical immunohistologic features with E-cadherin expression in para-carcinoma and GC tissues. D . Immunostaining for MMP2 was performed in GC; Magnifications, ×200.

    Article Snippet: The GC cells were grown and transfected with the NC or Rap1GAP CRISPR Activation Plasmid (Santa Cruz) using Lipofectamine 2000, according to the manufacturer's protocol.

    Techniques: Negative Control, Expressing, Staining, Immunostaining

    Differences in Rap1GAP, E-cadherin and MMP2 between the cancer tissues and para-carcinoma tissues

    Journal: Oncotarget

    Article Title: Low expression of Rap1GAP is associated with epithelial-mesenchymal transition (EMT) and poor prognosis in gastric cancer

    doi: 10.18632/oncotarget.14074

    Figure Lengend Snippet: Differences in Rap1GAP, E-cadherin and MMP2 between the cancer tissues and para-carcinoma tissues

    Article Snippet: The GC cells were grown and transfected with the NC or Rap1GAP CRISPR Activation Plasmid (Santa Cruz) using Lipofectamine 2000, according to the manufacturer's protocol.

    Techniques:

    Rap1GAP and E-cadherin protein in GC cells were notably lower expression compared to 293T cells by western blotting. Conversely, MMP2 was upregulated in GC cells (*P < 0.05 and **P < 0.01)

    Journal: Oncotarget

    Article Title: Low expression of Rap1GAP is associated with epithelial-mesenchymal transition (EMT) and poor prognosis in gastric cancer

    doi: 10.18632/oncotarget.14074

    Figure Lengend Snippet: Rap1GAP and E-cadherin protein in GC cells were notably lower expression compared to 293T cells by western blotting. Conversely, MMP2 was upregulated in GC cells (*P < 0.05 and **P < 0.01)

    Article Snippet: The GC cells were grown and transfected with the NC or Rap1GAP CRISPR Activation Plasmid (Santa Cruz) using Lipofectamine 2000, according to the manufacturer's protocol.

    Techniques: Expressing, Western Blot

    Correlations between the clinicopathologic variables with Rap1GAP, E-cadherin and MMP2

    Journal: Oncotarget

    Article Title: Low expression of Rap1GAP is associated with epithelial-mesenchymal transition (EMT) and poor prognosis in gastric cancer

    doi: 10.18632/oncotarget.14074

    Figure Lengend Snippet: Correlations between the clinicopathologic variables with Rap1GAP, E-cadherin and MMP2

    Article Snippet: The GC cells were grown and transfected with the NC or Rap1GAP CRISPR Activation Plasmid (Santa Cruz) using Lipofectamine 2000, according to the manufacturer's protocol.

    Techniques:

    Association between Rap1GAP with E-cadherin and MMP2

    Journal: Oncotarget

    Article Title: Low expression of Rap1GAP is associated with epithelial-mesenchymal transition (EMT) and poor prognosis in gastric cancer

    doi: 10.18632/oncotarget.14074

    Figure Lengend Snippet: Association between Rap1GAP with E-cadherin and MMP2

    Article Snippet: The GC cells were grown and transfected with the NC or Rap1GAP CRISPR Activation Plasmid (Santa Cruz) using Lipofectamine 2000, according to the manufacturer's protocol.

    Techniques:

    The cumulative overall survival differences between the patients with high and low levels of protein expression. The P value was obtained using the log-rank test of the difference. A . Rap1GAP; B . E-cadherin; C . MMP2.

    Journal: Oncotarget

    Article Title: Low expression of Rap1GAP is associated with epithelial-mesenchymal transition (EMT) and poor prognosis in gastric cancer

    doi: 10.18632/oncotarget.14074

    Figure Lengend Snippet: The cumulative overall survival differences between the patients with high and low levels of protein expression. The P value was obtained using the log-rank test of the difference. A . Rap1GAP; B . E-cadherin; C . MMP2.

    Article Snippet: The GC cells were grown and transfected with the NC or Rap1GAP CRISPR Activation Plasmid (Santa Cruz) using Lipofectamine 2000, according to the manufacturer's protocol.

    Techniques: Expressing

    Univariate Analysis for Overall Survival

    Journal: Oncotarget

    Article Title: Low expression of Rap1GAP is associated with epithelial-mesenchymal transition (EMT) and poor prognosis in gastric cancer

    doi: 10.18632/oncotarget.14074

    Figure Lengend Snippet: Univariate Analysis for Overall Survival

    Article Snippet: The GC cells were grown and transfected with the NC or Rap1GAP CRISPR Activation Plasmid (Santa Cruz) using Lipofectamine 2000, according to the manufacturer's protocol.

    Techniques:

    Multivariate Cox Proportional Hazards Analysis for Overall Survival

    Journal: Oncotarget

    Article Title: Low expression of Rap1GAP is associated with epithelial-mesenchymal transition (EMT) and poor prognosis in gastric cancer

    doi: 10.18632/oncotarget.14074

    Figure Lengend Snippet: Multivariate Cox Proportional Hazards Analysis for Overall Survival

    Article Snippet: The GC cells were grown and transfected with the NC or Rap1GAP CRISPR Activation Plasmid (Santa Cruz) using Lipofectamine 2000, according to the manufacturer's protocol.

    Techniques:

    The GC cells were grown and transfected with the negative control (NC) or Rap1GAP CRISPR Activation Plasmid. Overexpression Rap1GAP improved the expression of E-cadherin (A, B) and, inversely, suppressed the expression of MMP2 (A, B) compared to NC group in mRNA level and protein level. (*P < 0.05 and **P < 0.01).

    Journal: Oncotarget

    Article Title: Low expression of Rap1GAP is associated with epithelial-mesenchymal transition (EMT) and poor prognosis in gastric cancer

    doi: 10.18632/oncotarget.14074

    Figure Lengend Snippet: The GC cells were grown and transfected with the negative control (NC) or Rap1GAP CRISPR Activation Plasmid. Overexpression Rap1GAP improved the expression of E-cadherin (A, B) and, inversely, suppressed the expression of MMP2 (A, B) compared to NC group in mRNA level and protein level. (*P < 0.05 and **P < 0.01).

    Article Snippet: The GC cells were grown and transfected with the NC or Rap1GAP CRISPR Activation Plasmid (Santa Cruz) using Lipofectamine 2000, according to the manufacturer's protocol.

    Techniques: Transfection, Negative Control, CRISPR, Activation Assay, Plasmid Preparation, Over Expression, Expressing

    Overexpression Rap1GAP repressed the migration A . and invasion B . capacity of GC cells C . The graph shows the quantified data of the assay, migration (left) and invasion (right) (**P < 0.01).

    Journal: Oncotarget

    Article Title: Low expression of Rap1GAP is associated with epithelial-mesenchymal transition (EMT) and poor prognosis in gastric cancer

    doi: 10.18632/oncotarget.14074

    Figure Lengend Snippet: Overexpression Rap1GAP repressed the migration A . and invasion B . capacity of GC cells C . The graph shows the quantified data of the assay, migration (left) and invasion (right) (**P < 0.01).

    Article Snippet: The GC cells were grown and transfected with the NC or Rap1GAP CRISPR Activation Plasmid (Santa Cruz) using Lipofectamine 2000, according to the manufacturer's protocol.

    Techniques: Over Expression, Migration